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Preliminary study on preparation of cell-free system for protein expression

CHEN Haiqin, XU Zhinan, WANG Cheng, CEN Peilin, LIAO Yuhua

Frontiers of Chemical Science and Engineering 2008, Volume 2, Issue 2,   Pages 224-229 doi: 10.1007/s11705-008-0029-9

Abstract: In the new era of “Omics”, the traditional techniques of protein expression in vivo can not come up withThe cell-free protein synthesis system provides a new strategy of protein expression with advantagessystem are the key factors for the successful construction of cell-free protein expression system.The cell growth phase, the pressure for cell disruption and the storage condition of cell extracts werecell-free system with high expression level (Ca. 154 ?

Keywords： cell-free     regeneration     extract     information     successful construction    

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Cell-free systems in the new age of synthetic biology

Fernando Villarreal,Cheemeng Tan

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1,   Pages 58-65 doi: 10.1007/s11705-017-1610-x

Abstract: The advent of synthetic biology has ushered in new applications of cell-free transcription-translationThese cell-free systems are reconstituted using cellular proteins, and are amenable to modular controlHere, we discuss the historical advancement of cell-free systems, as well as their new applications incircuits and components, directed evolution of biomolecules, diagnosis of infectious diseases, and synthesisFinally, we present our vision on the future direction of cell-free synthetic biology.

Keywords： cell-free system     application    

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Enhanced Poly(ethylene terephthalate) Hydrolase Activity by Protein Engineering Article

Yuan Ma, Mingdong Yao, Bingzhi Li, Mingzhu Ding, Bo He, Si Chen, Xiao Zhou, Yingjin Yuan

Engineering 2018, Volume 4, Issue 6,   Pages 888-893 doi: 10.1016/j.eng.2018.09.007

Abstract: around the substrate-binding groove in order to create novel high-efficiency PETase mutants through proteinThe enzymatic activities of the R61A, L88F, and I179F mutants, which were obtained with a rapid cell-freeThus, this study has created enhanced artificial PETase enzymes through the rational protein engineering

Keywords： Polyesterase     PET degradation     Cell-free protein synthesis     Polyester     PETase    

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Application of liquid biopsy in precision medicine: opportunities and challenges

Junyun Wang, Shuang Chang, Guochao Li, Yingli Sun

Frontiers of Medicine 2017, Volume 11, Issue 4,   Pages 522-527 doi: 10.1007/s11684-017-0526-7

Abstract: Detecting circulating tumor cell (CTC) or circulating tumor DNA (ctDNA) in plasma or serum could serve

Keywords： liquid biopsy     circulating tumor cells     cell-free ctDNA     exosomes     precision medicine    

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Microcystin-LR biodegradation by

Chengbin XIAO, Hai YAN, Junfeng WANG, Wei WEI, Jun NING, Gang PAN

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 4,   Pages 526-532 doi: 10.1007/s11783-010-0261-7

Abstract: cyanobacterial cells collected from Lake Guishui and the biodegradation of MC-LR by both strain and cell-freeUSTB-05 containing 350 mg·L protein.

Keywords： microcystin-LR(MC-LR)     biodegradation     Sphingopyxis sp     USTB-05     cell-free extract    

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Cell surface protein engineering for high-performance whole-cell catalysts

Hajime Nakatani,Katsutoshi Hori

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1,   Pages 46-57 doi: 10.1007/s11705-017-1609-3

Abstract: Cell surface protein engineering facilitated by accumulation of information on genome and protein structureinvolves heterologous production and modification of cell surface proteins using genetic engineering, and is important for the development of high-performance whole-cell catalysts.In this field, cell surface display is a major technology by exposing target proteins, such as enzymes, on the cell surface using a carrier protein.

Keywords： cell surface engineering     surface display     whole-cell catalysts     bioprocess    

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Nitrogen-doped graphene approach to enhance the performance of a membraneless enzymatic biofuel cell

Alireza AHMADIAN YAZDI, Jie XU

Frontiers in Energy 2018, Volume 12, Issue 2,   Pages 233-238 doi: 10.1007/s11708-018-0529-3

Abstract: the anodic response to glucose detection leads to a higher overall performance of enzymatic biofuel cellThus, here we first synthesized N-graphene via a catalyst-free single-step thermal process, and made

Keywords： enzymatic fuel cell     nitrogen-doped graphene     reduced graphene oxide     catalyst-free synthesis    

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Effect of protein on PVDF ultrafiltration membrane fouling behavior under different pH conditions: interface

Xudong WANG,Miao ZHOU,Xiaorong MENG,Lei WANG,Danxi HUANG

Frontiers of Environmental Science & Engineering 2016, Volume 10, Issue 4, doi: 10.1007/s11783-016-0855-9

Abstract: predicts that the polar or Lewis acid–base (AB) interaction played a dominant role in the interfacial freesolution can have a significant impact on the process of membrane fouling by changing the AB component of free

Keywords： PVDF membrane     Membrane fouling     Adhesion force     Protein     Interfacial free energy    

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MEC-Empowered Non-Terrestrial Network for 6G Wide-Area Time-Sensitive Internet of Things Article

Chengxiao Liu, Wei Feng, Xiaoming Tao, Ning Ge

Engineering 2022, Volume 8, Issue 1,   Pages 96-107 doi: 10.1016/j.eng.2021.11.002

Abstract: Thus, we can build a non-terrestrial network (NTN) using a cell-free architecture.

Keywords： Cell-free Mobile edge computing     Non-terrestrial networks     Sixth-generation     Wide-area time-sensitive    

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Dynamic changes of protein corona compositions on the surface of zinc oxide nanoparticle in cell culture

Vo-Van Giau, Yoon-Hee Park, Kyu-Hwan Shim, Sang-Wook Son, Seong-Soo A. An

Frontiers of Chemical Science and Engineering 2019, Volume 13, Issue 1,   Pages 90-97 doi: 10.1007/s11705-018-1766-z

Abstract: into physiological environments and driven by energetics, they readily associate proteins forming a proteinsurface characteristics, affecting their interaction with cells due to conformational changes in adsorbed proteinapproach, we examined the composition of the PC for a set of zinc oxide nanoparticles (ZnONP) from cellthe surface of nanoparticles that may modify the structure, therefore the function of the adsorbed protein

Keywords： ZnONPs     nanoparticles     protein corona     ClueGO    

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Leucine Supplementation in a Chronically Protein-Restricted Diet Enhances Muscle Weight and PostprandialProtein Synthesis of Skeletal Muscle by Promoting the mTOR Pathway in Adult Rats

Bo Zhang, Licui Chu, Hong Liu, Chunyuan Xie, Shiyan Qiao, Xiangfang Zeng

Engineering 2017, Volume 3, Issue 5,   Pages 760-765 doi: 10.1016/J.ENG.2017.03.008

Abstract:

Low protein intake causes a decrease in protein deposition in most animal tissues.The purpose of this study was to investigate whether leucine supplementation would increase the synthesissynthesis rate, and the phosphorylation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1synthesis rate, and phosphorylation of both ribosomal protein S6 kinase 1 (S6K1) and 4E-BP1.improves body weight gain and increases muscle protein synthesis through mTOR activation.

Keywords： Low-protein diet     Leucine     Growth performance     Muscle weight     Protein synthesis     Adult rats    

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Insights into the Organotemplate-Free Synthesis of Zeolite Catalysts

Yeqing Wang,Qinming Wu,Xiangju Meng,Feng-Shou Xiao

Engineering 2017, Volume 3, Issue 4,   Pages 567-574 doi: 10.1016/J.ENG.2017.03.029

Abstract: briefly summarizes the role of organic templates and describes designed routes for the organotemplate-freesynthesis of zeolites, aided by zeolite seeds and zeolite seeds solution.synthesis of zeolite products, where Na+ exists as an alkali cation.This feature is very important in directing the synthesis of zeolite catalysts with controllable Si/Alratios under organotemplate-free conditions, and is thus important for the efficient design of zeolites

Keywords： Zeolite seed-directed synthesis     Organotemplate-free synthesis     Zeolite seeds solution     Si/Al ratios     Zeolite    

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Hepatitis B virus X protein upregulates tumor necrosis factor-α expression of rat mesangial cell

Hong-Zhu LU MD, Dan LIU BM, Qi-Hong FAN BM, Jian-Hua ZHOU MD,

Frontiers of Medicine 2010, Volume 4, Issue 1,   Pages 106-111 doi: 10.1007/s11684-010-0004-y

Abstract: Hepatitis B virus X protein (HBx), a 17-kd protein encoded by X gene of hepatitis B virus (HBV), hasThe aim of the study is to investigate the extracellular regulated protein kinases (ERKs) pathway ofHBx on glomerular mesangial cell (GMC) proliferation and tumor necrosis factor-α (TNF-α) expressionTNF-α mRNA expression was significantly decreased in U0126 group compared with U0126-free group.HBx upregulates TNF-α expression and induces cell proliferation of GMC line partly through ERK signal

Keywords： hepatitis B virus     X gene     glomerular mesangial cell line     extracellular regulated protein kinases     tumor    

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A facile synthesis of high activity cube-like Pt/carbon composites for fuel cell application

Reza B. MOGHADDAM, Samaneh SHAHGALDI, Xianguo LI

Frontiers in Energy 2017, Volume 11, Issue 3,   Pages 245-253 doi: 10.1007/s11708-017-0492-4

Abstract: High activity catalyst with simple low-cost synthesis is essential for fuel cell commercialization.In this study, a facile procedure for the synthesis of cube-like Pt nanoparticle (Pt ) composites withsupports is developed by mixing precursor of Pt with carbon supports in organic batches, hence, one pot synthesis

Keywords： synthesis     cube-like Pt     Pt/C composite     catalyst     impedance    

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Production of a polyclonal antibody to the VP26 nucleocapsid protein of white spot syndrome virus (wssv

Suchera LOYPRASERT-THANANIMIT, Akrapon SALEEDANG, Proespichaya KANATHARANA, Panote THAVARUNGKUL, Wilaiwan CHOTIGEAT

Frontiers of Chemical Science and Engineering 2012, Volume 6, Issue 2,   Pages 216-223 doi: 10.1007/s11705-012-1289-y

Abstract: assist in recognizing its host and assists the viral nucleocapsid to move toward the nucleus of the host cellThe recombinant VP26 protein (rVP26) was produced in (BL21), purified and used for immunizing rabbits

Keywords： recombinant protein     polyclonal antibody     label-free biosensor     impedance     white spot syndrome virus (WSSV    

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Title Author Date Type Operation

Preliminary study on preparation of cell-free system for protein expression

CHEN Haiqin, XU Zhinan, WANG Cheng, CEN Peilin, LIAO Yuhua

Journal Article

Cell-free systems in the new age of synthetic biology

Fernando Villarreal,Cheemeng Tan

Journal Article

Enhanced Poly(ethylene terephthalate) Hydrolase Activity by Protein Engineering

Yuan Ma, Mingdong Yao, Bingzhi Li, Mingzhu Ding, Bo He, Si Chen, Xiao Zhou, Yingjin Yuan

Journal Article

Application of liquid biopsy in precision medicine: opportunities and challenges

Junyun Wang, Shuang Chang, Guochao Li, Yingli Sun

Journal Article

Microcystin-LR biodegradation by

Chengbin XIAO, Hai YAN, Junfeng WANG, Wei WEI, Jun NING, Gang PAN

Journal Article

Cell surface protein engineering for high-performance whole-cell catalysts

Hajime Nakatani,Katsutoshi Hori

Journal Article

Nitrogen-doped graphene approach to enhance the performance of a membraneless enzymatic biofuel cell

Alireza AHMADIAN YAZDI, Jie XU

Journal Article

Effect of protein on PVDF ultrafiltration membrane fouling behavior under different pH conditions: interface

Xudong WANG,Miao ZHOU,Xiaorong MENG,Lei WANG,Danxi HUANG

Journal Article

MEC-Empowered Non-Terrestrial Network for 6G Wide-Area Time-Sensitive Internet of Things

Chengxiao Liu, Wei Feng, Xiaoming Tao, Ning Ge

Journal Article

Dynamic changes of protein corona compositions on the surface of zinc oxide nanoparticle in cell culture

Vo-Van Giau, Yoon-Hee Park, Kyu-Hwan Shim, Sang-Wook Son, Seong-Soo A. An

Journal Article

Leucine Supplementation in a Chronically Protein-Restricted Diet Enhances Muscle Weight and PostprandialProtein Synthesis of Skeletal Muscle by Promoting the mTOR Pathway in Adult Rats

Bo Zhang, Licui Chu, Hong Liu, Chunyuan Xie, Shiyan Qiao, Xiangfang Zeng

Journal Article

Insights into the Organotemplate-Free Synthesis of Zeolite Catalysts

Yeqing Wang,Qinming Wu,Xiangju Meng,Feng-Shou Xiao

Journal Article

Hepatitis B virus X protein upregulates tumor necrosis factor-α expression of rat mesangial cell

Hong-Zhu LU MD, Dan LIU BM, Qi-Hong FAN BM, Jian-Hua ZHOU MD,

Journal Article

A facile synthesis of high activity cube-like Pt/carbon composites for fuel cell application

Reza B. MOGHADDAM, Samaneh SHAHGALDI, Xianguo LI

Journal Article

Production of a polyclonal antibody to the VP26 nucleocapsid protein of white spot syndrome virus (wssv

Suchera LOYPRASERT-THANANIMIT, Akrapon SALEEDANG, Proespichaya KANATHARANA, Panote THAVARUNGKUL, Wilaiwan CHOTIGEAT

Journal Article